Produktbeschreibung
Mobicol Spin S-Columns for Nucleic Acid Purification. Mobicol Spin S-columns are pre-packed with S-200, S-300, or S-400 Sephacryl® HR matrices. Sephacryl® High Resolution chromatography resins are highly versatile resins that offer a wide range of fractionation capabilities. They allow fast and reproducible purification of proteins, polysaccharides, nucleic acids, and other macromolecules by size exclusion chromatography. The Mobicol Spin S-columns separate molecules by differences in size. The columns combine the effectiveness of gel filtration with speed of centrifugation. The pore size of the filled-in matrix determines which molecules are small enough to enter the pores of the matrix beads and which molecules are too large. In this way, compounds larger than the pore size are excluded from the resin and located in the void volume. These molecules move quickly through the matrix bed when the column is centrifuged. Molecules smaller than the pore size, like hydrated salt ions, do enter the pores of the matrix beads and are held back. Applications: • Buffer exchange • Nucleotide removal • Plasmid, oligonucleotide, and PCR reaction purification • Removal of unincorporated dyes or dye terminators. (Sephacryl® is a registered trademark of Cytiva)
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